[摘要] 目锟斤拷 探锟斤拷锟斤拷锟酵革拷锟阶诧拷锟斤拷(HBV)锟斤拷锟节革拷染锟侥匡拷锟杰伙拷锟斤拷锟斤拷锟斤拷锟斤拷 应锟斤拷PC R锟斤拷锟斤拷锟斤拷锟�59锟斤拷锟斤拷锟酵革拷锟阶诧拷锟斤拷锟斤拷锟芥抗原(HBsAg)锟斤拷锟斤拷锟叫革拷锟斤拷锟斤拷水锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤及锟斤拷锟斤拷 锟斤拷锟斤拷锟斤拷血锟斤拷锟斤拷HBV DNA(锟叫撅拷锟斤拷)锟斤拷10锟斤拷锟斤拷锟酵革拷锟阶诧拷锟斤拷锟斤拷志锟斤拷(HBVM)锟斤拷锟皆碉拷锟斤拷锟斤拷锟叫革拷 锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷为锟斤拷锟斤拷锟介。锟斤拷锟斤拷锟斤拷锟斤拷锟介化ABC染色锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷懈锟教ワ拷锟斤拷锟街拷械锟紿BsAg锟斤拷 锟斤拷锟酵革拷锟阶猴拷锟侥匡拷原(HBcAg)锟斤拷锟斤拷锟斤拷锟绞★拷锟斤拷锟� (1)锟叫撅拷锟斤拷锟叫革拷锟斤拷锟斤拷水锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤、 锟斤拷锟斤拷锟斤拷锟斤拷血锟斤拷锟斤拷HBVDNA锟斤拷锟斤拷锟绞分憋拷为47.5锟斤拷(28锟斤拷59)锟斤拷52.5锟斤拷(31锟斤拷59)锟斤拷45.8锟斤拷( 27锟斤拷59)锟斤拷锟斤拷锟斤拷锟斤拷锟叫革拷锟斤拷锟斤拷水锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤、锟斤拷锟斤拷锟斤拷锟斤拷血锟斤拷锟叫撅拷未锟斤拷锟紿BVDNA锟斤拷(2 )锟叫撅拷锟斤拷锟叫革拷胎锟斤拷锟斤拷织锟斤拷HBsAg锟斤拷HBcAg锟斤拷锟斤拷锟斤拷锟绞ｏ拷锟斤拷锟街筹拷锟斤拷锟斤拷膜细锟斤拷(76.3锟斤拷锟斤拷59. 3锟斤拷)锟斤拷锟斤拷锟斤拷锟斤拷细锟斤拷(72.9锟斤拷锟斤拷55.9锟斤拷)锟斤拷锟斤拷毛锟斤拷锟斤拷细锟斤拷(62.7锟斤拷锟斤拷50.8锟斤拷)锟斤拷锟斤拷毛毛细 血锟斤拷锟斤拷皮细锟斤拷(52.5锟斤拷锟斤拷44.1锟斤拷)锟斤拷锟轿递硷拷锟斤拷锟斤拷锟狡ｏ拷锟斤拷锟斤拷锟斤拷锟斤拷4锟斤拷锟叫革拷胎锟斤拷锟斤拷织锟斤拷HB sAg锟斤拷HBcAg锟侥分诧拷锟斤拷锟斤拷锟斤拷锟截碉拷锟洁反锟斤拷锟叫撅拷锟斤拷锟叫革拷锟斤拷32锟斤拷锟斤拷膜细锟斤拷锟叫硷拷锟紿Bs Ag锟斤拷H BcAg锟斤拷锟斤拷锟斤拷锟斤拷锟叫革拷胎锟斤拷锟斤拷织锟叫碉拷HBsAg锟斤拷HBcAg锟斤拷锟斤拷锟轿拷恪ｏ拷锟斤拷锟� 锟叫革拷血锟斤拷HBV锟斤拷 要锟斤拷通锟斤拷锟斤拷染胎锟教碉拷锟斤拷胎锟斤拷锟斤拷染锟斤拷锟斤拷也锟斤拷锟杰达拷锟斤拷胎锟斤拷锟斤拷锟斤拷母锟饺就撅拷锟斤拷锟� 锟斤拷锟酵革拷锟阶诧拷锟斤拷(HBV)锟斤拷染锟斤拷锟斤拷一锟斤拷锟斤拷锟斤拷锟皆癸拷锟斤拷锟斤拷锟斤拷锟斤拷锟解，锟揭癸拷锟截达拷锟斤拷锟酵革拷锟阶革拷 锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷群锟斤拷锟斤拷锟酵革拷锟阶诧拷锟斤拷锟斤拷锟芥抗原(HBsAg)锟斤拷锟斤拷锟绞高达拷10锟斤拷锟斤拷锟较★拷锟斤拷锟斤拷锟斤拷锟叫撅拷 锟斤拷锟斤拷锟斤拷HBV锟斤拷锟节革拷染锟斤拷HBV锟斤拷锟斤拷要锟斤拷锟斤拷途锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷夜锟斤拷锟饺猴拷写锟斤拷诖锟斤拷锟紿BV携锟斤拷锟斤拷 锟斤拷锟斤拷要原锟津。碉拷目前锟皆癸拷锟节革拷染锟侥伙拷锟斤拷锟斤拷锟斤拷全锟斤拷系统锟斤拷锟叫撅拷锟斤拷锟斤拷锟叫撅拷锟斤拷锟斤拷PCR锟斤拷锟斤拷 锟斤拷锟紿BaAs锟斤拷锟斤拷锟叫革拷锟斤拷锟斤拷水锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤及锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷血锟斤拷HBVDNA锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟介化 锟斤拷锟斤拷锟教ワ拷锟斤拷锟街拷锟紿BsAg锟斤拷锟斤拷锟酵革拷锟阶猴拷锟侥匡拷原(HBcAg)锟侥分诧拷状锟斤拷锟斤拷旨锟斤拷探锟斤拷HBV锟斤拷 锟节革拷染锟侥匡拷锟杰伙拷锟斤拷锟斤拷 锟斤拷锟斤拷锟诫方锟斤拷 一锟斤拷锟叫撅拷锟斤拷锟斤拷 为锟姐东省锟斤拷锟斤拷锟叫革拷锟阶憋拷锟斤拷院锟斤拷锟斤拷1997锟斤拷10锟斤拷锟斤拷1999锟斤拷3锟斤拷锟斤拷锟铰凤拷锟斤拷锟斤拷懈锟斤拷锟斤拷锟� 锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟叫革拷锟斤拷锟斤拷锟斤按锟节硷拷椋︼拷锟矫革拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟�(ELISA)锟斤拷锟斤拷锟紿BV血锟斤拷 锟斤拷志锟斤拷(HBVM)锟斤拷锟叫撅拷锟斤拷为HBsAg锟斤拷锟斤拷锟叫革拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷(59锟斤拷)锟斤拷锟斤拷锟斤拷锟斤拷为HBVM锟斤拷锟皆碉拷 锟斤拷锟斤拷锟叫革拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷(10锟斤拷)锟斤拷锟斤拷选锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟绞凤拷锟斤拷锟斤拷锟斤拷锟较诧拷症锟斤拷 锟斤拷锟斤拷症锟斤拷锟斤拷锟斤拷锟叫革拷锟斤拷锟戒、锟叫诧拷锟轿硷拷锟斤拷锟斤拷时胎锟斤拷冉希锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟�(P>0.05)锟斤拷 锟斤拷锟斤拷锟叫撅拷锟斤拷锟斤拷 1锟斤拷PCR锟斤拷锟紿BVDNA锟斤拷锟叫革拷锟斤拷院锟斤拷未锟斤拷膜前锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷取锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷 锟斤拷锟斤拷装锟斤拷2ml锟斤拷锟斤拷锟斤拷水锟皆癸拷锟叫ｏ拷锟斤拷锟斤拷锟斤拷毯锟斤拷锟绞憋拷锟斤拷锟斤拷膜时锟斤拷锟斤拷取锟斤拷水锟斤拷锟斤拷锟斤拷血 锟斤拷染锟斤拷胎锟斤拷锟斤拷锟斤拷锟斤拷诮锟教ワ拷锟斤拷顺锟斤拷锟窖�3ml锟斤拷锟斤拷锟斤拷血锟藉。锟斤拷锟叫标本锟斤拷-20锟芥保锟芥，统一 锟斤拷锟紿BV DNA锟斤拷HBV DNA锟斤拷PCR锟斤拷锟斤拷约锟斤拷锟斤拷苫锟斤拷锟斤拷锟斤拷锕わ拷坦锟剿撅拷峁╋拷锟斤拷锟斤拷锟斤拷细锟剿� 锟斤拷锟斤拷锟斤拷锟斤拷 2锟斤拷锟斤拷锟斤拷锟介化ABC染色锟斤拷锟斤拷锟教ワ拷锟斤拷锟街拷锟紿BsAg锟斤拷HBcAg锟斤拷胎锟斤拷锟斤拷锟斤拷锟斤拷锟�30min锟斤拷 锟街憋拷取胎锟斤拷胎锟斤拷锟芥及母锟斤拷锟斤拷锟斤拷织约1 cm锟斤拷l cm锟斤拷2cm锟斤拷小锟斤拷锟斤拷10锟斤拷锟斤拷醛锟斤拷液锟教讹拷锟斤拷 锟斤拷锟斤拷石锟斤拷锟斤拷锟今，斤拷锟斤拷5锟斤拷m锟斤拷片锟斤拷锟斤拷锟斤拷锟介化锟斤拷锟斤拷锟矫匡拷HBcAg锟斤拷小锟斤拷HBsAg锟斤拷染色锟皆硷拷 锟叫硷拷DAB锟斤拷色药锟叫撅拷锟斤拷锟斤拷锟戒汉锟斤拷士锟斤拷锟斤拷锟斤工锟斤拷锟斤拷锟睫癸拷司锟斤拷每锟斤拷染色锟斤拷锟斤拷锟斤拷HBsAg锟斤拷锟斤拷 锟斤拷HBcAg锟斤拷锟斤拷尸锟斤拷锟斤拷锟斤拷锟街拷锟斤拷锟斤拷远锟斤拷眨锟斤拷锟紿BVM锟斤拷锟斤拷锟皆碉拷锟斤拷锟铰诧拷胎锟斤拷锟斤拷织锟斤拷锟斤拷锟皆讹拷 锟秸ｏ拷同时锟斤拷PBS锟斤拷锟斤拷一锟斤拷锟秸白讹拷锟秸★拷锟斤拷细锟斤拷锟斤拷锟斤拷细锟斤拷锟斤拷锟斤拷锟斤拷锟截伙拷色为强锟斤拷锟斤拷染色锟斤拷 锟叫碉拷锟截伙拷色为锟斤拷锟斤拷染色锟斤拷浅锟截伙拷色为锟斤拷锟斤拷锟斤拷染色锟斤拷 锟斤拷锟斤拷锟斤拷锟节革拷染锟斤拷锟斤拷锟� 锟斤拷锟斤拷锟斤拷锟斤拷血锟斤拷HBV DNA锟斤拷锟斤拷锟斤拷锟轿拷锟斤拷诟锟饺撅拷锟� 锟斤拷锟� 一锟斤拷锟斤拷锟斤拷锟叫革拷锟斤拷水锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤及锟斤拷锟斤拷锟斤拷锟斤拷血锟斤拷HBV DNA锟斤拷锟斤拷锟绞比斤拷 锟叫撅拷锟斤拷锟叫革拷锟斤拷水锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤、锟斤拷锟斤拷锟斤拷锟斤拷血锟斤拷锟叫撅拷锟斤拷锟斤拷HBV DNA锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷 锟叫革拷锟斤拷锟斤拷应锟斤拷织锟叫撅拷未锟斤拷锟斤拷HBV DNA锟斤拷 锟斤拷锟斤拷锟斤拷锟斤拷锟叫革拷胎锟斤拷锟斤拷织锟斤拷HBsAg锟斤拷HBcAg锟斤拷锟斤拷锟绞比斤拷 (1)锟叫撅拷锟斤拷锟叫革拷胎锟斤拷锟斤拷织HBsAg锟斤拷锟斤拷锟斤拷为81.4锟斤拷(48锟斤拷59)锟斤拷HBcAg锟斤拷锟斤拷锟斤拷为61.0 锟斤拷(36锟斤拷59)锟斤拷胎锟斤拷锟斤拷织锟斤拷HBsAg锟绞诧拷锟斤拷一锟斤拷锟截伙拷色锟斤拷锟斤拷状染色锟斤拷锟姐泛锟斤拷锟斤拷锟斤拷胎锟斤拷锟斤拷 织锟斤拷锟斤拷细锟斤拷锟斤拷锟斤拷锟叫★拷HBsAg锟斤拷锟斤拷锟绞筹拷锟街筹拷锟斤拷锟斤拷膜细锟斤拷锟斤拷锟斤拷锟斤拷细锟斤拷锟斤拷锟斤拷毛锟斤拷锟斤拷细锟斤拷锟斤拷 锟斤拷毛毛细血锟斤拷锟斤拷皮细锟斤拷锟斤拷锟斤拷膜锟斤拷皮细锟斤拷锟斤拷锟轿递硷拷锟斤拷锟斤拷锟狡★拷锟节诧拷锟斤拷锟斤拷毛锟斤拷隙锟缴硷拷HBs Ag锟斤拷锟斤拷染色锟斤拷HBsAg锟斤拷染细锟斤拷锟斤拷锟狡达拷锟斤拷趴锟阶达拷植锟斤拷锟�(2)胎锟斤拷锟斤拷织锟斤拷HBcAg锟斤拷锟斤拷染 色锟绞撅拷锟饺匡拷锟斤拷状锟斤拷锟街诧拷锟斤拷锟斤拷染细锟斤拷锟斤拷细锟斤拷锟斤拷锟叫ｏ拷锟斤拷染细锟斤拷锟斤拷锟叫★拷锟阶达拷锟缴拷诜植锟斤拷锟� HBcAg锟斤拷锟斤拷锟绞筹拷锟街筹拷锟斤拷锟斤拷膜细锟斤拷锟斤拷锟斤拷锟斤拷细锟斤拷锟斤拷锟斤拷毛锟斤拷锟斤拷细锟斤拷锟斤拷锟斤拷毛毛细血锟斤拷锟斤拷皮细 锟斤拷锟斤拷锟轿递硷拷锟斤拷锟斤拷锟狡★拷(3)锟斤拷锟斤拷锟斤拷胎锟斤拷锟斤拷织锟斤拷未锟斤拷锟斤拷HBsAg锟斤拷HBcAg锟斤拷锟斤拷染色细锟斤拷锟斤拷锟斤拷 锟斤拷锟斤拷为锟姐。(4)锟叫撅拷锟斤拷锟叫革拷锟斤拷锟斤拷4锟斤拷HBsAg锟斤拷HBcAg锟斤拷胎锟斤拷锟斤拷织锟叫的分诧拷锟斤拷锟斤拷锟街筹拷锟斤拷锟斤拷 毛毛细血锟斤拷锟斤拷皮细锟斤拷锟斤拷锟斤拷锟斤拷细锟斤拷锟斤拷锟斤拷膜细锟斤拷锟斤拷锟轿递硷拷锟斤拷锟斤拷锟狡ｏ拷锟斤拷锟斤拷细锟斤拷锟斤拷色强锟斤拷锟斤拷 锟斤拷锟斤拷锟斤拷锟斤拷 锟斤拷锟斤拷 学锟斤拷锟斤拷锟秸憋拷锟斤拷为锟斤拷HBV锟斤拷锟节革拷染锟侥伙拷锟斤拷为锟斤拷胎锟教革拷染锟斤拷1987锟疥，Lin锟斤拷[1]锟斤拷锟斤拷 锟斤拷锟介化PAP锟斤拷锟斤拷锟斤拷锟�32锟斤拷HBsAg锟斤拷HBeAg双锟斤拷锟斤拷锟叫革拷锟斤拷锟铰凤拷锟斤拷锟教ワ拷蹋锟斤拷锟斤拷未锟斤拷锟紿B sAg锟斤拷Lucifora锟斤拷[2锟斤拷3]锟街憋拷锟斤拷锟斤拷锟斤拷锟介化锟斤拷锟斤拷锟斤拷锟�12锟斤拷HBsAg锟斤拷症状携锟斤拷锟斤拷锟斤拷锟铰凤拷锟斤拷 锟斤拷胎锟斤拷锟斤拷织锟斤拷锟斤拷锟斤拷HBsAg锟斤拷HBcAg锟斤拷锟斤拷锟斤拷锟斤拷毛血锟斤拷锟斤拷皮细锟斤拷锟叫★拷锟斤拷锟斤拷锟斤拷水平锟斤拷[4]锟斤拷 锟斤拷锟斤拷锟介化ABC锟斤拷锟斤拷锟斤拷锟紿BsAg锟斤拷锟斤拷锟叫革拷锟斤拷锟铰凤拷锟斤拷胎锟斤拷锟斤拷织锟斤拷锟斤拷锟斤拷HBsAg锟斤拷HBcAg锟斤拷锟斤拷锟斤拷 胎锟斤拷锟斤拷织锟斤拷锟斤拷细锟斤拷锟叫ｏ拷锟揭硷拷锟斤拷始锟斤拷锟斤拷锟较革拷锟斤拷锟侥匡拷锟斤拷锟侥は革拷锟斤拷锟斤拷锟斤拷锟斤拷锟较革拷锟斤拷锟斤拷锟矫拷锟� 锟斤拷细锟斤拷锟斤拷锟斤拷毛毛细血锟斤拷锟斤拷皮细锟斤拷锟斤拷锟斤拷锟轿递硷拷锟斤拷锟斤拷锟狡★拷锟斤拷锟斤拷锟斤拷锟斤拷[5]锟斤拷原位锟斤拷锟斤拷锟接斤拷 锟斤拷锟斤拷锟解定锟斤拷24锟斤拷HBsAg锟斤拷HBeAg双锟斤拷锟斤拷锟叫革拷锟斤拷锟铰凤拷锟斤拷锟教ワ拷锟斤拷锟街拷锟斤拷锟斤拷锟斤拷示锟斤拷HBVDN A锟斤拷要锟街诧拷锟斤拷胎锟斤拷锟斤拷膜细锟斤拷锟斤拷锟斤拷锟斤拷毛锟斤拷锟斤拷细锟斤拷未锟斤拷锟街凤拷锟斤拷锟斤拷锟较憋拷锟斤拷锟斤拷锟叫撅拷锟斤拷锟斤拷锟斤拷锟� 锟斤拷锟斤拷锟斤拷为锟剿ｏ拷锟斤拷锟斤拷锟斤拷PCR锟斤拷锟斤拷锟斤拷锟斤拷锟介化ABC锟斤拷锟斤拷锟�59锟斤拷HBsAg锟斤拷锟斤拷锟斤拷锟铰诧拷锟斤拷锟斤拷胎锟斤拷锟斤拷 织锟斤拷锟斤拷锟斤拷锟绞撅拷锟紿BsAg锟斤拷HBcAg锟斤拷锟斤拷锟绞分憋拷锟斤拷81.4锟斤拷(48锟斤拷59)锟斤拷61.0锟斤拷(36锟斤拷59)锟斤拷H BsAg锟斤拷HBcAg锟斤拷锟斤拷省锟斤拷锟斤拷锟较革拷锟斤拷锟秸硷拷锟斤拷锟斤拷锟较革拷锟斤拷锟缴匡拷锟斤拷锟斤拷锟侥は革拷锟斤拷锟斤拷锟斤拷锟斤拷锟较革拷锟� 锟斤拷锟斤拷毛锟斤拷锟斤拷细锟斤拷锟斤拷锟斤拷毛毛细血锟斤拷锟斤拷皮细锟斤拷锟斤拷锟轿递硷拷锟斤拷锟斤拷毛毛细血锟斤拷锟斤拷皮细锟斤拷锟斤拷染3 1锟斤拷锟斤拷锟斤拷锟斤拷24锟斤拷锟斤拷锟斤拷锟剿癸拷锟节革拷染锟斤拷锟斤拷染锟斤拷为77.4锟斤拷锟斤拷HBV锟缴革拷染胎锟斤拷锟斤拷织锟叫的革拷锟斤拷细 锟斤拷锟斤拷锟斤拷染锟斤拷胎锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷平锟饺憋拷锟斤拷锟侥斤拷锟斤拷锟斤拷啤锟紿BV锟斤拷胎锟教癸拷锟节革拷染锟侥伙拷锟狡ｏ拷锟斤拷锟斤拷 锟斤拷锟叫革拷锟斤拷毛锟斤拷隙血锟叫碉拷HBV锟斤拷染锟斤拷膜细锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷染胎锟斤拷细锟斤拷锟斤拷直锟斤拷锟斤拷毛毛细血 锟斤拷锟斤拷皮细锟斤拷锟斤拷锟斤拷HBV直锟接革拷染锟斤拷毛锟斤拷锟斤拷细锟斤拷锟斤拷锟斤拷毛毛细血锟斤拷锟斤拷皮细锟斤拷锟斤拷锟接讹拷锟斤拷锟斤拷胎 锟斤拷锟斤拷染锟斤拷 锟斤拷锟叫撅拷锟斤拷锟斤拷锟街ｏ拷4锟斤拷锟叫革拷胎锟斤拷锟斤拷织锟叫ｏ拷HBsAg锟斤拷HBcAg锟斤拷锟斤拷细锟斤拷锟斤拷锟斤拷目锟斤拷锟斤拷毛毛 细血锟斤拷锟斤拷皮细锟斤拷锟斤拷锟斤拷膜细锟斤拷锟金渐硷拷锟劫ｏ拷锟斤拷锟斤拷细锟斤拷锟斤拷色强锟斤拷锟金渐硷拷锟斤拷锟斤拷锟斤拷锟斤拷2锟斤拷锟斤拷血 HBV DNA锟斤拷锟皆★拷锟斤拷示HBV锟斤拷锟饺革拷染胎锟斤拷锟斤拷锟斤拷锟轿革拷染胎锟教革拷锟斤拷细锟斤拷锟斤拷锟斤拷4锟斤拷胎锟斤拷锟斤拷膜锟斤拷 皮细锟斤拷锟斤拷锟紿BsAg锟斤拷HBcAg锟斤拷锟斤拷水锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷HBVDNA锟斤拷锟斤拷锟皆ｏ拷说锟斤拷锟斤拷锟杰达拷锟节撅拷锟斤拷锟斤拷 锟斤拷锟斤拷锟叫革拷染锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟叫碉拷HBV锟斤拷锟轿革拷染胎膜锟斤拷锟斤拷水锟斤拷胎锟斤拷锟斤拷然锟斤拷锟饺咎ワ拷锟斤拷锟� 织锟侥革拷锟斤拷细锟斤拷锟斤拷 20锟斤拷锟斤拷80锟斤拷锟斤拷穑锟斤拷锟斤拷锟窖э拷锟斤拷群锟街な碉拷锟斤拷锟斤拷锟紿BV锟斤拷染锟竭革拷锟斤拷锟斤拷锟斤拷细锟斤拷锟酵撅拷锟斤拷 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